FURIN / PCSK3 Antibody

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ProSci
Product Code: PSI-43-351
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-351-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with 43-351 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-351 (1ug/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with 43-351 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-351 (1ug/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
FURIN, furin (paired basic amino acid cleaving enzyme), FUR, PACE, PCSK3, SPC1, FES upstream region, dibasic processing enzyme, furin, furin, membrane associated receptor protein, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein), proprotein convertase subtilisin/kexin type 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx. 110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Background References:
  • Jin W, Wang X, Millar JS, Quertermous T, Rothblat GH, Glick JM, Rader DJHepatic proprotein convertases modulate HDL metabolism
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RTKRDVYQEPTDP
NCBI Gene ID #:
5045
NCBI Official Name:
furin (paired basic amino acid cleaving enzyme)
NCBI Official Symbol:
FURIN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002560.1
Protein GI Number:
120611
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
Swissprot #:
P09958