COPA Antibody

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ProSci
Product Code: PSI-43-187
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-187-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-187 (0.5ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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43-187 (0.5ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
COPA, coatomer protein complex, subunit alpha, FLJ26320, HEP-COP, alpha coat protein, xenin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 140kDa band observed in Human Prostate lysates (calculated MW of 138kDa according to NP_001091868.1and NP_004362.2). Recommended concentration: 0.5-2ug/ml. An additional band of 52kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Background References:
  • Kano F, Yamauchi S, Yoshida Y, Watanabe-Takahashi M, Nishikawa K, Nakamura N, Murata M, Yip1A regulates the COPI-independent retrograde transport from the Golgi complex to the ER.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: CTRASNLENST
NCBI Gene ID #:
1314
NCBI Official Name:
coatomer protein complex, subunit alpha
NCBI Official Symbol:
COPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 140kDa
Protein Accession #:
NP_001091868.1, NP_004362.2
Protein GI Number:
148536853
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Growth Factor Signal Transduction Growth Factor
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001091868.1; NP_004362.2).
Swissprot #:
P53621