PARP12 Antibody
| Code | Size | Price |
|---|
| PSI-43-116-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PARP12, poly (ADP-ribose) polymerase family, member 12, HGNC:21919, FLJ22693, PARP-12, ZC3H1, ZC3HDC1, zinc finger CCCH type domain containing 1, zinc finger CCCH-type domain containing 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.
Background References:
- Katoh M, Katoh M. Identification and characterization of human TIPARP gene within the CCNL amplicon at human chromosome 3q25.31.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-NAHDIKNKSSAP
NCBI Gene ID #:
64761
NCBI Official Name:
poly (ADP-ribose) polymerase family, member 12
NCBI Official Symbol:
PARP12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_073587.1
Protein GI Number:
12232413
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Obesity
Swissprot #:
Q9H0J9