Makorin Antibody

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ProSci
Product Code: PSI-43-114
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-114-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with 43-114 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-114 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with 43-114 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-114 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
MKRN1, makorin, ring finger protein 1, HGNC:7112, RNF61, makorin ring finger protein 1, FLJ21334
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Background References:
  • Kim JH, Park SM, Kang MR, Oh SY, Lee TH, Muller MT, Chung IK. Ubiquitin ligase MKRN1 modulates telomere length homeostasis through a proteolysis of hTERT.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-VTKEESEKEQTAVET
NCBI Gene ID #:
23608
NCBI Official Name:
makorin ring finger protein 1
NCBI Official Symbol:
MKRN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_038474.1, NP_001138597.1
Protein GI Number:
223468620
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Homeostasis,Apoptosis Homeostasis
SPECIFICITY:
This antibody may cross react with MKRN4 (52.9kDa).
Swissprot #:
Q9UHC7