CCAR2 / DBC1 Antibody

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ProSci
Product Code: PSI-43-639
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-639-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-639 (0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-639 (0.5ug/ml) staining of HEK293 (A) and MCF7 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CCAR2, cell cycle and apoptosis regulator 2, DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1, p30 DBC protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 140kDa band observed in nuclear lysates of cell lines HEK293, MCF7 and NIH3T3 (calculated MW of 103kDa according to Human NP_066997.3 and Mouse NP_666167.3). The observed molecular weight is explained by its acetylation (Zheng et al, Mol Cell Biol. 2013 Dec;33(24):4960-70. PMID: 24126058). Recommended concentration: 0.5-2ug/ml.
Background References:
  • Kim SH, Kim JH, Yu EJ, Lee KW, Park CK.The overexpression of DBC1 in esophageal squamous cell carcinoma correlates with poor prognosis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESHNRFSATEVTNK
NCBI Gene ID #:
57805
NCBI Official Name:
cell cycle and apoptosis regulator 2
NCBI Official Symbol:
CCAR2
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 140kDa
Protein Accession #:
NP_066997.3
Protein GI Number:
24432106
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
Q8N163