AOC3 / VAP1 Antibody

ProSci
Product Code: PSI-43-199
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-199-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-199 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-199 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
AOC3, amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in lysates of cell line A549 (calculated MW of 84.6kDa according to NP_003725.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Kiss J, Jalkanen S, Fülöp F, Savunen T, Salmi M, Ischemia-reperfusion injury is attenuated in VAP-1-deficient mice and by VAP-1 inhibitors.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: ESQAPKTIRDAFC
NCBI Gene ID #:
8639
NCBI Official Name:
amine oxidase, copper containing 3
NCBI Official Symbol:
AOC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_003725.1
Protein GI Number:
4502119
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
Q16853