GNAI2 Antibody

ProSci
Product Code: PSI-43-712
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-712-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-712 (2ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-712 (2ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
GNAI2, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1, GTP-binding regulatory protein Gi alpha-2 chain, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide-binding protein G(i) subunit alpha-2, guanine nucleotide-binding protein G(i), alpha-2 subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 40.5kDa according to NP_002061.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid and Tonsil. Recommended concentration: 5ug/ml.
Background References:
  • Kremer KN, Kumar A, Hedin KE.G alpha i2 and ZAP-70 mediate RasGRP1 membrane localization and activation of SDF-1-induced T cell functions.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PEYTGANKYDE
NCBI Gene ID #:
2771
NCBI Official Name:
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
NCBI Official Symbol:
GNAI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 38kDa
Protein Accession #:
NP_002061.1, NP_001159897.1
Protein GI Number:
4504041
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Signal Transduction,Immunology Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_002061.1; NP_001159897.1).
Swissprot #:
P04899