histamine H3 receptor Antibody

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ProSci
Product Code: PSI-43-162
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-162-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-162 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-162 (0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
HRH3, histamine receptor H3, GPCR97, HH3R, G protein-coupled receptor 97, G-protein coupled receptor 97, H3R, OTTHUMP00000031459, histamine H3 receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in lysates of cell line A549 (calculated MW of 48.7kDa according to NP_009163.2). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Kuramasu A, Sukegawa J, Sato T, Sakurai E, Watanabe T, Yanagisawa T, Yanai K.The hydrophobic amino acids in putative helix 8 in carboxy-terminus of histamine H(3) receptor are involved in receptor-G-protein coupling.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SVTRAVSYRAQQGDT
NCBI Gene ID #:
11255
NCBI Official Name:
histamine receptor H3
NCBI Official Symbol:
HRH3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 48kDa
Protein Accession #:
NP_009163.2
Protein GI Number:
194018562
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience,Signal Transduction,Neuroscience Signal Transduction
Swissprot #:
Q9Y5N1