AIFM1 Antibody

Product Code: PSI-43-657

Supplier: ProSci

Code	Size	Price

PSI-43-657-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-657 (0.01ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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3 / 6

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43-657 (0.03ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

5 / 6

43-657 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DA

6 / 6

43-657 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DA

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Documents

Further Information

Additional Names:

AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor

Application Note:

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence:?Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10ug/ml.

Background References:

Lewis EM, Wilkinson AS, Jackson JS, Mehra R, Varambally S, Chinnaiyan AM, Wilkinson JC.The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells.

Buffer:

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.

Concentration:

500 ug/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Expected Species Reactivity based on sequence homology: Dog, Cow

Immunogen:

The immunogen for this antibody is: C-NEVAKLFNIHED

NCBI Gene ID #:

9131

NCBI Official Name:

apoptosis-inducing factor, mitochondrion-associated, 1

NCBI Official Symbol:

AIFM1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Approx 70kDa

Protein Accession #:

NP_004199.1, NP_665811.1, NP_665812.1, NP_001124318.1

Protein GI Number:

22202631

Purification:

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Research Area:

Homeostasis,Homeostasis

SPECIFICITY:

This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).

Swissprot #:

O95831-2