SFRP4 Antibody

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ProSci
Product Code: PSI-42-066
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-066-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-066 (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-066 (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SFRP4, secreted frizzled-related protein 4, FRP-4, FRPHE, MGC26498, secreted frizzled-related protein 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4ug/ml.
Background References:
  • Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1;21(23):3110-22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: TNSSCQCPHILPHQD
NCBI Gene ID #:
6424
NCBI Official Name:
secreted frizzled-related protein 4
NCBI Official Symbol:
SFRP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003005.2
Protein GI Number:
170784838
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell
SPECIFICITY:
.
Swissprot #:
Q6FHJ7