MLC1 Antibody

ProSci
Product Code: PSI-43-286
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-286-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-286 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-286 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
MLC1, megalencephalic leukoencephalopathy with subcortical cysts 1, LVM, MLC, VL, membrane protein MLC1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (L?pez-Hern?ndez et al, Hum Mol Genet. 2011 Aug 15;20(16):3266-77.PMID:21624973). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Background References:
  • L?pez-Hern?ndez T, Sirisi S, Capdevila-Nortes X, Montolio M, Fern?ndez-Due?as V, Scheper GC, van der Knaap MS, Casquero P, Ciruela F, Ferrer I, Nunes V, Est?vez R.Molecular mechanisms of MLC1 and GLIALCAM mutations in megalencephalic leukoencephalopathy with subcortical cysts.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SDSANILDEVPFPAR
NCBI Gene ID #:
23209
NCBI Official Name:
megalencephalic leukoencephalopathy with subcortical cysts 1
NCBI Official Symbol:
MLC1
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_055981.1
Protein GI Number:
14589896
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,
SPECIFICITY:
Reported variants represent identical protein: NP_631941.1, NP_055981.1.
Swissprot #:
Q15049