RE1-silencing transcription factor Antibody

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ProSci
Product Code: PSI-43-692
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-692-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-692 (0.3ug/ml) staining of HEK293 (A) and HeLa (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-692 (0.3ug/ml) staining of HEK293 (A) and HeLa (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
REST, RE1-silencing transcription factor, NRSF, XBR, RE1-silencing transcription factor variant E1a/E2/E3/E4c, RE1-silencing transcription factor variant E1a/E2/E3/E5, RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4, RE1-silencing transcription factor variant E1a/E2/E4, RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j, RE1-silencing transcription factor variant E1b/E2/E3/E5, RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i, RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4, RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4, RE1-silencing transcription factor variant E1c/E2/E3/E5, RE1-silencing transcription factor variant E1c/E2g/E3/E4, neural-restrictive silencer factor, neuron restrictive silencer factor, repressor binding to the X2 box
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 140kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122kDa according to NP_005603.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, Yang TH, Kim HM, Drake D, Liu XS, Bennett DA, Colai?covo MP, Yankner BA.REST and stress resistance in ageing and Alzheimer's disease.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PETPGAEDKGKSSK
NCBI Gene ID #:
5978
NCBI Official Name:
RE1-silencing transcription factor
NCBI Official Symbol:
REST
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 140kDa
Protein Accession #:
NP_005603.3
Protein GI Number:
301897977
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell,Stem Cell
SPECIFICITY:
Reported variants represent identical protein: NP_001180437.1, NP_005603.3
Swissprot #:
Q13127