cytokeratin 20 Antibody

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ProSci
Product Code: PSI-43-166
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-166-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-166 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-166 (0.03ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1). Recommended concentration: 0.01-0.03ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA as a capture antibody in combiation with reporter antibody EB11745.
Background References:
  • Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, BorrebaecSATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-NSRHTVNYGSDLT
NCBI Gene ID #:
54474
NCBI Official Name:
keratin 20
NCBI Official Symbol:
KRT20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 49kDa
Protein Accession #:
NP_061883.1
Protein GI Number:
27894337
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
P35900