FYB Antibody

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ProSci
Product Code: PSI-43-248
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-248-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-248 (0.2ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-248 (0.2ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
FYB, FYN binding protein (FYB-120/130) , ADAP, PRO0823, SLAP-130, FYN-binding protein (FYB-120/130), adhesion and degranulation-promoting adaptor protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 90kDa band observed in lysates of cell line MOLT4 (calculated MW of 90.6kDa according to NP_001456.3). Recommended concentration: 0.2-0.6ug/ml.
Background References:
  • Medeiros RB, Burbach BJ, Mueller KL, Srivastava R, Moon JJ, Highfill S, Peterson EJ, Shimizu Y.Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SEEKPDKEPKP
NCBI Gene ID #:
2533
NCBI Official Name:
FYN binding protein (FYB-120/130)
NCBI Official Symbol:
FYB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_001456.3, NP_955367.1, NP_001230022.1
Protein GI Number:
42476118
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Immunology
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_001456.3; NP_955367.1; NP_001230022.1).
Swissprot #:
O15117