TNNI2 Antibody

ProSci
Product Code: PSI-43-288
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-288-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-288 (0.03ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-288 (0.03ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
TNNI2, troponin I type 2 (skeletal, fast), AMCD2B, DA2B, FSSV, fsTnI, fast-twitch skeletal muscle troponin I, troponin I fast twitch 2, troponin I, fast skeletal muscle, troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform, troponin I, skeletal, fast
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 21.3kDa according to NP_003273.1). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Moran CM, Garriock RJ, Miller MK, Heimark RL, Gregorio CC, Krieg PA.Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: KRNRAITARRQHLKS-C
NCBI Gene ID #:
7136
NCBI Official Name:
troponin I type 2 (skeletal, fast)
NCBI Official Symbol:
TNNI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 25kDa
Protein Accession #:
NP_003273.1, NP_001139313.1
Protein GI Number:
4507621
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Swissprot #:
P48788