MECL1 Antibody

ProSci
Product Code: PSI-42-017
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-017-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-017 (0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-017 (0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9kDa according to NP_002792.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows efined staining in a subset of cells. Recommended concentration, 5-10ug/ml.
Background References:
  • Moschonas A, Kouraki M, Knox PG, Thymiakou E, Kardassis D, Eliopoulos AG. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Molecular and Cellular Biology 2008 Oct 28 (20): 6208-22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PTEPVKRSGRYH
NCBI Gene ID #:
5699
NCBI Official Name:
proteasome (prosome, macropain) subunit, beta type, 10
NCBI Official Symbol:
PSMB10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002792.1
Protein GI Number:
4506191
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Chemokines & Cytokines,Innate Immunity
SPECIFICITY:
.
Swissprot #:
P40306