ARNT Antibody

ProSci
Product Code: PSI-43-504
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-504-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-504 (2ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-504 (2ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ARNT, aryl hydrocarbon receptor nuclear translocator, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, OTTHUMP00000218217, OTTHUMP00000218218, OTTHUMP00000218220, class E basic helix-loop-helix protein 2, dioxin receptor, nuclear translocator, hypoxia-inducible factor 1, beta subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 90kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6kDa according to NP_001659.1). Recommended concentration: 2-4ug/ml.
Background References:
  • Otsubo K, Kanegane H, Eguchi M, Eguchi-Ishimae M, Tamura K, Nomura K, Abe A, Ishii E, Miyawaki T.ETV6-ARNT fusion in a patient with childhood T lymphoblastic leukemia.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CNDKERFARSDDEQS
NCBI Gene ID #:
405
NCBI Official Name:
aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol:
ARNT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_001659.1,
Protein GI Number:
114163
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
Swissprot #:
P27540