Thrombospondin Antibody

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ProSci
Product Code: PSI-43-469
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-469-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-469 (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-469 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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43-469 (5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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43-469 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
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43-469 (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-469 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol
43-469 (5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
43-469 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Documents

Further Information

Additional Names:
THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Background References:
  • Pan MR, Chang HC, Chuang LY, Hung WC.The nonsteroidal anti-inflammatory drug NS398 reactivates SPARC expression via promoter demethylation to attenuate invasiveness of lung cancer cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: NRIPESGGDNSVFD-C
NCBI Gene ID #:
7057
NCBI Official Name:
thrombospondin 1
NCBI Official Symbol:
THBS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003237.2
Protein GI Number:
40317626
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
Swissprot #:
P07996