CAPN1 Antibody

ProSci
Product Code: PSI-43-259
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-259-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-259 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 2

43-259 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
CAPN1, calpain 1, (mu/I) large subunit, CANP, CANP1, CANPL1, muCANP, muCL, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 catalytic subunit, calpain-1 large subunit, cell proliferation-inducing gene 30 protein, cell proliferation-inducing protein 30, micromolar-calpain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in Human Kidney lysates (calculated MW of 81.9kDa according to NP_005177.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
  • Panigrahi AK, Zhang N, Mao Q, Pati D.Calpain-1 cleaves Rad21 to promote sister chromatid separation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NASRARSEQFINLR
NCBI Gene ID #:
823
NCBI Official Name:
calpain 1, (mu/I) large subunit
NCBI Official Symbol:
CAPN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 80kDa
Protein Accession #:
NP_005177.2
Protein GI Number:
115574
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Signal Transduction,
SPECIFICITY:
Reported variants represent identical protein: NP_001185797.1, NP_005177.2, NP_001185798.1.
Swissprot #:
P07384