HMGI-C / HMGA2 Antibody

ProSci
Product Code: PSI-43-134
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-134-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-134 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-134 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
HMGA2, high mobility group AT-hook 2, BABL, HMGI-C, HMGIC, LIPO, STQTL9, High-mobility group protein HMGI-C, high-mobility group (nonhistone chromosomal) protein isoform I-C
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3ug/ml.
Background References:
  • Patel UA, Bandiera A, Manfioletti G, Giancotti V, Chau KY, Crane-Robinson CExpression and cDNA cloning of human HMGI-C phosphoprotein
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KAAQKKAEATGEK
NCBI Gene ID #:
8091
NCBI Official Name:
high mobility group AT-hook 2
NCBI Official Symbol:
HMGA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 18kDa
Protein Accession #:
NP_003474.1, NP_003475.1
Protein GI Number:
4504431
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003474.1; NP_003475.1) and is not expected to cross-react with HMGA1.
Swissprot #:
P52926