NUMBL Antibody

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ProSci
Product Code: PSI-43-109
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-109-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Numbl with C-terminal MYC tag probed with 43-109 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-109 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Numbl with C-terminal MYC tag probed with 43-109 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-109 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
NUMBL, numb homolog (Drosophila)-like, NBL, CAG3A, CTG3a, NUMBR, NUMB-R, TNRC23, numb (Drosophila) homolog-like, NUMBLIKE, TNRC23, numb homolog-like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing Human Numbl and DYKDDDDK tagged), a band of approx. 85kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Background References:
  • Petersen PH, Zou K, Hwang JK, Jan YN, Zhong W.Progenitor cell maintenance requires numb and numblike during mouse neurogenesis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: SRSAAASGGPRRPE-C
NCBI Gene ID #:
9253
NCBI Official Name:
numb homolog (Drosophila)-like
NCBI Official Symbol:
NUMBL
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
Protein Accession #:
NP_004747.1
Protein GI Number:
10863899
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Obesity,Signal Transduction,
Swissprot #:
Q9Y6R0