PIAS2 Antibody

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ProSci
Product Code: PSI-43-141
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-141-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-141 (1ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-141 (1ug/ml) staining of Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PIAS2, protein inhibitor of activated STAT, 2, MGC102682, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, miz, Msx-interacting-zinc finger, protein inhibitor of activated STAT X, zinc finger, MIZ-type containing 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Mononucleocytes lysates (calculated MW of 63.4kDa according to NP_775298.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Saito M, Novak U, Piovan E, Basso K, Sumazin P, Schneider C, Crespo M, Shen Q, Bhagat G, Califano A, Chadburn A, Pasqualucci L, Dalla-Favera R, BCL6 suppression of BCL2 via Miz1 and its disruption in diffuse large B cell lymphoma.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: PQQVREICISRD
NCBI Gene ID #:
9063
NCBI Official Name:
protein inhibitor of activated STAT, 2
NCBI Official Symbol:
PIAS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_775298.1, NP_004662.2
Protein GI Number:
119621890
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).
Swissprot #:
O75928