MSI2 / musashi-2 Antibody

ProSci
Product Code: PSI-43-615
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-615-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-615 (0.1ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-615 (0.1ug/ml) staining of Kelly lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
MSI2, musashi RNA-binding protein 2, MSI2H, RNA-binding protein Musashi homolog 2, musashi homolog 2, musashi-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell line Kelly (calculated MW of 35.2kDa according to NP_620412.1). Recommended concentration: 0.1-0.3ug/ml. Approx 37+39kDa bands observed in fetal Mouse Brain lysates (calculated MW of 36.9kDa according to Mouse NP_473384.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Background References:
  • Sakakibara S, Nakamura Y, Satoh H, Okano H.Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-SPDSLRDYFSK
NCBI Gene ID #:
124540
NCBI Official Name:
musashi homolog 2 (Drosophila)
NCBI Official Symbol:
MSI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 37kDa
Protein Accession #:
NP_620412.1, NP_733839.1
Protein GI Number:
20373175
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell,Stem Cell
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_620412.1; NP_733839.1).
Swissprot #:
Q96DH6