IL3RA / CD123 Antibody

ProSci
Product Code: PSI-43-665
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-665-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-665 (2ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-665 (2ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
IL3RA, interleukin 3 receptor, alpha (low affinity), CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, CD123 antigen, IL-3 receptor alpha SP2 isoform, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, interleukin-3 receptor subunit alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3kDa according to NP_002174.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
Background References:
  • Shi J, Ikeda K, Maeda Y, Shinagawa K, Ohtsuka A, Yamamura H, Tanimoto M.Identification of CD123+ myeloid dendritic cells as an early-stage immature subset with strong tumoristatic potential.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: RQQYECLHYKTD
NCBI Gene ID #:
3563
NCBI Official Name:
interleukin 3 receptor, alpha (low affinity)
NCBI Official Symbol:
IL3RA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 43kDa
Protein Accession #:
NP_002174.1, NP_001254642.1
Protein GI Number:
13324710
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_002174.1; NP_001254642.1).
Swissprot #:
P26951