NME1 Antibody

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ProSci
Product Code: PSI-43-626
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-626-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-626 (0.01ug/ml) staining of A549 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-626 (0.01ug/ml) staining of A549 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
NME1, AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1, non-metastatic cells 1, protein (NM23A) expressed in, NDP kinase A, OTTHUMP00000174772
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 20+18kDa bands observed in lysates of cell lines A549, HeLa and HepG2 (calculated MW of 19.7kDa according to NP_937818.1 and 17.1kDa according to NP_000260.1). Recommended concentration: 0.01-0.03ug/ml.
Background References:
  • Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Su Kim D, Choi YD, Moon M, Kang S, Lim JB, Kim KM, Park KM, Cho NH.Composite three-marker assay for early detection of kidney cancer.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: DYTSCAQNWIYE
NCBI Gene ID #:
4830
NCBI Official Name:
NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol:
NME1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 20 + 18kDa
Protein Accession #:
NP_937818.1, NP_000260.1
Protein GI Number:
127981
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_937818.1; NP_000260.1).
Swissprot #:
P15531