IGFBP1 Antibody

ProSci
Product Code: PSI-43-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-247-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
43-247 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

43-247 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
IGFBP1, insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IBP-1, IGF-binding protein 1, IGFBP-1, OTTHUMP00000159561, OTTHUMP00000207990, OTTHUMP00000214348, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, insulin-like growth factor-binding protein 1, placental protein 12
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1). Recommended concentration: 1-3ug/ml. An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
Background References:
  • Sugita S, Morishita Y, Kano J, Furuya S, Shiba-Ishii A, Noguchi M.IGFBP-1 is expressed specifically in ovarian clear cell adenocarcinoma.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-STYDGSKALHVTNIK
NCBI Gene ID #:
3484
NCBI Official Name:
insulin-like growth factor binding protein 1
NCBI Official Symbol:
IGFBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_000587.1
Protein GI Number:
124055
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Signal Transduction,
Swissprot #:
P08833