cytochrome P450 1A1 Antibody

ProSci
Product Code: PSI-43-597
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-597-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-597 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-597 (0.3ug/ml) staining of A549 (A) and MCF7 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CYP1A1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, CYPIA1, OTTHUMP00000183469, aryl hydrocarbon hydroxylase, cytochrome P1-450, dioxin-inducible, cytochrome P450 1A1, cytochrome P450 form 6, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1, cytochrome P450-C, cytochrome P450-P1, flavoprotein-linked monooxygenase, xenobiotic monooxygenase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Sulem P, Gudbjartsson DF, Geller F, Prokopenko I, Feenstra B, Aben KK, Franke B, den Heijer M, Kovacs P, Stumvoll M, M?gi R, Yanek LR, Becker LC, Boyd HA, Stacey SN, Walters GB, Jonasdottir A, Thorleifsson G, Holm H, Gudjonsson SA, Rafnar T, Bj?rnsdottirSequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CQEKQLDENANVQ
NCBI Gene ID #:
1543
NCBI Official Name:
cytochrome P450, family 1, subfamily A, polypeptide 1
NCBI Official Symbol:
CYP1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 85-90kDa
Protein Accession #:
NP_000490.1
Protein GI Number:
4503199
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Signal Transduction,Cancer Signal Transduction Membrane Membrane
SPECIFICITY:
This antibody is not expected to cross-react with the similar P450 1A2
Swissprot #:
P04798