Cullin 3 Antibody

ProSci
Product Code: PSI-43-558
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-558-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-558 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-558 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CUL3, cullin 3, KIAA0617
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 90kDa band observed in Human Heart lysates (calculated MW of 88.9kDa according to NP_003581.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Sumara I, Quadroni M, Frei C, Olma MH, Sumara G, Ricci R, Peter M.A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-AQVTGSNTRKHILQ
NCBI Gene ID #:
8452
NCBI Official Name:
cullin 3
NCBI Official Symbol:
CUL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 90kDa
Protein Accession #:
NP_003581.1
Protein GI Number:
12643396
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
Q13618