CD3-ZETA / CD247 Antibody

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ProSci
Product Code: PSI-43-634
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-634-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-634 (0.1ug/ml) staining of Jurkat (A) and (0.3ug/ml) MOLT4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-634 (0.1ug/ml) staining of Jurkat (A) and (0.3ug/ml) MOLT4 (B) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CD247, CD247 molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, TCR zeta chain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16-17kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6kDa according to NP_000725.1). Recommended concentration: 0.1-1ug/ml.
Background References:
  • Teruel M, McKinney C, Balsa A, Pascual-Salcedo D, Rodriguez-Rodriguez L, Ortiz AM, G?mez-Vaquero C, Gonz?lez-Gay MA, Smith M, Witte T, Merriman T, Lie BA, Martin J.Association of CD247 polymorphisms with rheumatoid arthritis: a replication study and a meta-analysis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-KNPQEGLYNELQKD
NCBI Gene ID #:
919
NCBI Official Name:
CD247 molecule
NCBI Official Symbol:
CD247
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 16 + 17kDa
Protein Accession #:
NP_932170.1, NP_000725.1
Protein GI Number:
23830999
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_932170.1; NP_000725.1).
Swissprot #:
P20963