dUTPase / DUT Antibody

ProSci
Product Code: PSI-43-711
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-711-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-711 (0.1ug/ml) staining of HEK293 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-711 (0.1ug/ml) staining of HEK293 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
DUT, deoxyuridine triphosphatase, dUTPase, dUTP nucleotidohydrolase, dUTP pyrophosphatase, deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Tonsil. Recommended concentration: 5ug/ml.
Background References:
  • T?th J, Varga B, Kov?cs M, M?ln?si-Csizmadia A, V?rtessy BG.Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QLRFARLSEHATAPT
NCBI Gene ID #:
1854
NCBI Official Name:
deoxyuridine triphosphatase
NCBI Official Symbol:
DUT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 20kDa
Protein Accession #:
NP_001020419.1, NP_001939.1, NP_001020420.1
Protein GI Number:
70906441
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Immunology
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_001020419.1; NP_001939.1; NP_001020420.1).
Swissprot #:
P33316