MMP14 Antibody

ProSci
Product Code: PSI-43-321
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-321-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-321 (1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-321 (1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MMP14, matrix metallopeptidase 14 (membrane-inserted), 1, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, matrix metalloproteinase 14 (membrane-inserted), matrix metalloproteinase-14, membrane type 1 metalloprotease, membrane-type matrix metalloproteinase 1, membrane-type-1 matrix metalloproteinase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Ueda M, Yamashita Y, Takehara M, Terai Y, Kumagai K, Ueki K, Kanda K, Yamaguchi H, Akise D, Hung YC, Ueki M.Survivin gene expression in endometriosis.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESATPLRFREVP
NCBI Gene ID #:
4323
NCBI Official Name:
matrix metallopeptidase 14 (membrane-inserted)
NCBI Official Symbol:
MMP14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_004986.1
Protein GI Number:
317373419
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
Swissprot #:
P50281