EAAT1 / GLAST1 Antibody

ProSci
Product Code: PSI-43-236
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-236-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-236 (2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-236 (2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SLC1A3, solute carrier family 1 (glial high affinity glutamate transporter), member 3, EA6, EAAT1, FLJ25094, GLAST, GLAST1, GLAST-1, OTTHUMP00000116002, excitatory amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 1, solute carrier family 1 member 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3ug/ml.
Background References:
  • Vandenberg RJ, Handford CA, Campbell EM, Ryan RM, Yool AJ.Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DNETEKPIDSETK
NCBI Gene ID #:
6507
NCBI Official Name:
solute carrier family 1 (glial high affinity glutamate transporter), member 3
NCBI Official Symbol:
SLC1A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_004163.3, NP_001160167.1
Protein GI Number:
169790839
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Obesity, Signal Transduction
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Swissprot #:
P43003