EAAT1 / GLAST1 Antibody
| Code | Size | Price |
|---|
| PSI-43-235-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
SLC1A3, solute carrier family 1 (glial high affinity glutamate transporter), member 3, EA6, EAAT1, FLJ25094, GLAST, GLAST1, GLAST-1, OTTHUMP00000116002, excitatory amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 1, solute carrier family 1 member 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 0.1-0.3ug/ml.
An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Vandenberg RJ, Handford CA, Campbell EM, Ryan RM, Yool AJ.Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-RSFKVPIQANET
NCBI Gene ID #:
6507
NCBI Official Name:
solute carrier family 1 (glial high affinity glutamate transporter), member 3
NCBI Official Symbol:
SLC1A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_004163.3, NP_001160167.1
Protein GI Number:
169790839
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Swissprot #:
P43003