PNP Antibody
| Code | Size | Price |
|---|
| PSI-43-273-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PNP, purine nucleoside phosphorylase, NP, PRO1837, PUNP, inosine phosphorylase, purine-nucleoside:orthophosphate ribosyltransferase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of Human Liver, Erythrocytes and of cell lines Daudi, Jurkat and K562 (calculated MW of 32.1kDa according to NP_000261.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 5ug/ml.
Background References:
- Vareed SK, et al.Metabolites of purine nucleoside phosphorylase (NP) in serum have the potential to delineate pancreatic adenocarcinoma.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SGQNPLRGPNDE
NCBI Gene ID #:
4860
NCBI Official Name:
purine nucleoside phosphorylase
NCBI Official Symbol:
PNP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30kDa
Protein Accession #:
NP_000261.2
Protein GI Number:
157168362
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Obesity,Signal Transduction,
Swissprot #:
P00491