FCER1A Antibody

ProSci
Product Code: PSI-43-625
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-625-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-625 (0.3ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-625 (0.3ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
FCER1A, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, FCE1A, FcERI, Fc IgE receptor, alpha polypeptide, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, igE Fc receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Pripheral Blood Mononucleocytes lysates (calculated MW of 29.6kDa according to NP_001992.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ganguly et al, J Biol Chem. 2007 Nov 9;282(45):32758-64.PMID: 17728245). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Vasudev M, Cheung DS, Pincsak H, Li SH, Yan K, Simpson P, Dasu T, Grayson MH.Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TGKVWQLDYESEP
NCBI Gene ID #:
2205
NCBI Official Name:
Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
NCBI Official Symbol:
FCER1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 50kDa
Protein Accession #:
NP_001992.1
Protein GI Number:
119865
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P12319