myotilin Antibody

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ProSci
Product Code: PSI-43-201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-201-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-201 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-201 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MYOT, myotilin, LGMD1, LGMD1A, TTID, 57 kDa cytoskeletal protein, OTTHUMP00000159430, OTTHUMP00000223460, OTTHUMP00000223461, myofibrillar titin-like Ig domains protein, titin immunoglobulin domain protein (myotilin)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 55.4kDa according to NP_006781.1. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • von Nandelstadh P, Soliymani R, Baumann M, Carpen O.Analysis of myotilin turnover provides mechanistic insight into the role of myotilinopathy-causing mutations.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DSQQHNSEHARLQ
NCBI Gene ID #:
9499
NCBI Official Name:
myotilin
NCBI Official Symbol:
MYOT
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_006781.1, NP_001129412.1
Protein GI Number:
5803106
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Immunology,Stem Cell,
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_006781.1; NP_001129412.1).
Swissprot #:
Q9UBF9