polo-like kinase 3 Antibody

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ProSci
Product Code: PSI-43-290
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-290-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-290 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-290 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PLK3, polo-like kinase 3, CNK, FNK, PRK, FGF-inducible kinase, PLK-3, cytokine-inducible kinase, cytokine-inducible serine/threonine-protein kinase, proliferation-related kinase, serine/threonine-protein kinase PLK3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 71.6kDa according to NP_004064.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows vesicular staining in the cystoplasm of collective tubules. Recommended concentration, 5-10ug/ml.
Background References:
  • Wang J, Beauchemin M, Bertrand R.Bcl-xL phosphorylation at Ser49 by polo kinase 3 during cell cycle progression and checkpoints.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PDRLPISSCVTVPD
NCBI Gene ID #:
1263
NCBI Official Name:
polo-like kinase 3
NCBI Official Symbol:
PLK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_004064.2
Protein GI Number:
41872374
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience, Obesity, Signal Transduction
Swissprot #:
Q9H4B4