PGK1 Antibody

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ProSci
Product Code: PSI-43-549
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-549-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-549 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-549 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PGK1, phosphoglycerate kinase 1, MIG10, PGKA, PRP 2, cell migration-inducing gene 10 protein, primer recognition protein 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in lysates of cell lines A432, HEK293, HeLa and HepG2 (calculated MW of 44.6kDa according to NP_000282.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, Pienta KJ, Taichman RS.Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SKKYAEAVTRAKQ
NCBI Gene ID #:
5230
NCBI Official Name:
phosphoglycerate kinase 1
NCBI Official Symbol:
PGK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_000282.1
Protein GI Number:
4505763
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
Swissprot #:
P00558