VEGFA Antibody

ProSci
Product Code: PSI-43-168
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-168-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 3
43-168 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 3
3 / 3

43-168 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OTTHUMP00000224154, OTTHUMP00000224423, OTTHUMP00000224424, OTTHUMP00000224425, OTTHUMP00000224426, OTTHUMP00000224427, OTTHUMP00000224428, OTTHUMP00000224429, OTTHUMP00000224430, vascular permeability factor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney and Lung) for this particular batch.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml.Immunofluorescence:?Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10ug/ml.
Background References:
  • Weiss TW, Simak R, Kaun C, Rega G, Pfl?ger H, Maurer G, Huber K, Wojta J.Oncostatin M and IL-6 induce u-PA and VEGF in prostate cancer cells and correlate in vivo.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-RIKPHQGQHIGE
NCBI Gene ID #:
7422
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
VEGFA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_001020537.2, NP_003367.4, NP_001020538.2, NP_001020539.2, etc.
Protein GI Number:
17380528
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize all reported isoforms.
Swissprot #:
P15692