BMP1 Antibody

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ProSci
Product Code: PSI-43-642
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-642-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-642 (1ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-642 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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43-642 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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43-642 (1ug/ml) staining of Human Heart (A) and Kidney (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-642 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-642 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
BMP1, bone morphogenetic protein 1, OI13, PCOLC, PCP, PCP2, TLD, mammalian tolloid protein, procollagen C-endopeptidase, procollagen C-proteinase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5ug/ml.
Background References:
  • Wermter C, H?wel M, Hintze V, Bombosch B, Aufenvenne K, Yiallouros I, St?cker W.The protease domain of procollagen C-proteinase (BMP1) lacks substrate selectivity, which is conferred by non-proteolytic domains.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DTIVPKYEVNGVK
NCBI Gene ID #:
649
NCBI Official Name:
bone morphogenetic protein 1
NCBI Official Symbol:
BMP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 110kDa
Protein Accession #:
NP_001190.1, NP_006120.1
Protein GI Number:
13124688
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction,Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).
Swissprot #:
P13497