GNL3 Antibody

ProSci
Product Code: PSI-43-686
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-686-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-686 (2ug/ml) staining of HeLa (A)HepG2 (B) and K562 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-686 (2ug/ml) staining of HeLa (A)HepG2 (B) and K562 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
GNL3, guanine nucleotide binding protein-like 3 (nucleolar), C77032, E2IG3, NNP47, NS, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide-binding protein-like 3, novel nucleolar protein 47, nucleolar GTP-binding protein 3, nucleostemin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 65+75kDa bands observed in nuclear lysates of cell lines HeLa, HepG2 and K562 (calculated MW of 62.0kDa according to NP_055181.3). The observed 75kDa band likely represents the acetylated form. Recommended concentration: 1-3ug/ml.
Background References:
  • Yoshida R, Fujimoto T, Kudoh S, Nagata M, Nakayama H, Shinohara M, Ito T.Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EKNNAQSIRAIKGPH
NCBI Gene ID #:
26354
NCBI Official Name:
guanine nucleotide binding protein-like 3 (nucleolar)
NCBI Official Symbol:
GNL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 65 + 75kDa
Protein Accession #:
NP_055181.3, NP_996561.1
Protein GI Number:
7657048
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis,Homeostasis
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.
Swissprot #:
Q9BVP2