HIC1 Antibody
Code | Size | Price |
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PSI-43-339-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, hic-1, hypermethylated in cancer 1 protein, zinc finger and BTB domain-containing protein 29
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1ug/ml.
Background References:
- Yu J, Liu P, Cui X, Sui Y, Ji G, Guan R, Sun D, Ji W, Liu F, Liu A, Zhao Y, Yu Y, Jin Y, Bai J, Geng J, Xue Y, Qi J, Lee KY, Fu S.Identification of novel subregions of LOH in gastric cancer and analysis of the HIC1 and TOB1 tumor suppressor genes in these subregions.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: NTHCAELYASGP
NCBI Gene ID #:
3090
NCBI Official Name:
hypermethylated in cancer 1
NCBI Official Symbol:
HIC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006488.2, NP_001091672.1
Protein GI Number:
61676186
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis,Cancer,Cell Cycle,Transcription,Apoptosis Cancer Cell Cycle Transcription
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Swissprot #:
Q14526-2