LDHA Antibody

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ProSci
Product Code: PSI-43-515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-515-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-515 (0.3ug/ml) staining of Heart (A), Skeletal Muscle (B) and Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-515 (0.3ug/ml) staining of Heart (A), Skeletal Muscle (B) and Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
LDHA, lactate dehydrogenase A, GSD11, LDH1, LDHM, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in Human Heart, Skeletal Muscle and Kidney lysates (calculated MW of 36.7kDa according to NP_005557.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Zhang Y, Zhang X, Wang X, Gan L, Yu G, Chen Y, Liu K, Li P, Pan J, Wang J, Qin S.Inhibition of LDH-A by lentivirus-mediated small interfering RNA suppresses intestinal-type gastric cancer tumorigenicity through the downregulation of Oct4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SDLVKVTLTSE
NCBI Gene ID #:
3939
NCBI Official Name:
lactate dehydrogenase A
NCBI Official Symbol:
LDHA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 35kDa
Protein Accession #:
NP_005557.1, NP_001128711.1, NP_001158886.1
Protein GI Number:
5031857
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer,Cancer
SPECIFICITY:
This antibody is expected to recognize isoform 1, 2 and 3 (NP_005557.1; NP_001128711.1; NP_001158886.1).
Swissprot #:
P00338