creatine kinase M-type Antibody

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ProSci
Product Code: PSI-43-272
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-272-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-272 (0.01ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-272 (0.01ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CKM, creatine kinase, muscle, CKMM, M-CK, creatine kinase M chain, creatine kinase M-type, creatine kinase-M
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Background References:
  • Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M.Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: NTHNKFKLNYKPEE-C
NCBI Gene ID #:
1158
NCBI Official Name:
creatine kinase, muscle
NCBI Official Symbol:
CKM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 45kDa
Protein Accession #:
NP_001815.2
Protein GI Number:
21536288
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Obesity, Signal Transduction
Swissprot #:
P06732