POU2F2 / OCT2 Antibody

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ProSci
Product Code: PSI-43-195
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-195-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-195 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-195 (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
POU2F2, POU class 2 homeobox 2, OCT2, OTF2, Oct-2, POU domain, class 2, transcription factor 2, homeobox protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Tonsil and Mouse Thymus lysates (calculated MW of 49.5kDa according to NP_002689.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pelis et al, Am J Physiol Renal Physiol. 2006 May;290(5):F1118-26. PMID: 16368738). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Duan H, Xiang H, Ma L, Boxer LM, Functional long-range interactions of the IgH 3' enhancers with the bcl-2 promoter region in t(14;18) lymphoma cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: KQGLDSPSEHTDTER-C
NCBI Gene ID #:
5452
NCBI Official Name:
POU class 2 homeobox 2
NCBI Official Symbol:
POU2F2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 60kDa
Protein Accession #:
NP_002689.1
Protein GI Number:
4505959
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology,Signal Transduction,Transcription,Immunology Signal Transduction Transcription
Swissprot #:
P09086-4