TNNI1 Antibody

ProSci
Product Code: PSI-43-291
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-291-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-291 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43-291 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
TNNI1, troponin I type 1 (skeletal, slow), SSTNI, TNN1, troponin I, slow skeletal muscle, troponin I, slow-twitch isoform
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 21.7kDa according to NP_003272.3). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Engel PL, Kobayashi T, Biesiadecki B, Davis J, Tikunova S, Wu S, Solaro RJ.Identification of a region of troponin I important in signaling cross-bridge-dependent activation of cardiac myofilaments.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: PEVERKPKITASRK-C
NCBI Gene ID #:
7135
NCBI Official Name:
troponin I type 1 (skeletal, slow)
NCBI Official Symbol:
TNNI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 25kDa
Protein Accession #:
NP_003272.3
Protein GI Number:
56682969
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Other
Swissprot #:
P19237