SRRT / ARS2 Antibody

ProSci
Product Code: PSI-43-680
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-680-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-680 (0.1ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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43-680 (0.1ug/ml) staining of Daudi (A) and Mouse Spleen (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
SRRT, serrate RNA effector molecule homolog (Arabidopsis), ARS2, ASR2, serrate, arsenate resistance protein 2, arsenate resistance protein ARS2, arsenite resistance protein, arsenite-resistance protein 2, serrate RNA effector molecule homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings with different antibodies from other commercial sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml.
Background References:
  • Gruber JJ, Olejniczak SH, Yong J, La Rocca G, Dreyfuss G, Thompson CB.Ars2 promotes proper replication-dependent histone mRNA 3' end formation.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DPRAIVEYRDLDAPD
NCBI Gene ID #:
51593
NCBI Official Name:
serrate, RNA effector molecule
NCBI Official Symbol:
SRRT
NCBI Organism:
Homo Sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 140kDa
Protein Accession #:
NP_056992.4, NP_001122324.1, NP_001122325.1, NP_001122326.1
Protein GI Number:
58331218
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody is expected to recognize all reported isoform (NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1).
Swissprot #:
Q9BXP5