AP2A1 Antibody

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ProSci
Product Code: PSI-43-204
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-204-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-204 (0.01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-204 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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43-204 (0.01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-204 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit alpha-1, alpha-adaptin A, alpha1-adaptin, clathrin assembly protein complex 2 alpha-A large chain, clathrin-associated/assembly/adaptor protein, large, alpha 1, plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Background References:
  • Kitagawa Y, Kameoka M, Shoji-Kawata S, Iwabu Y, Mizuta H, Tokunaga K, Fujino M, Natori Y, Yura Y, Ikuta K.Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ELEPPAPESPMALLADPAPAAD
NCBI Gene ID #:
160
NCBI Official Name:
adaptor-related protein complex 2, alpha 1 subunit
NCBI Official Symbol:
AP2A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 110kDa
Protein Accession #:
NP_055018.2
Protein GI Number:
19913414
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology, Stem Cell
SPECIFICITY:
This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Swissprot #:
O95782