RFX5 Antibody

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ProSci
Product Code: PSI-43-250
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-250-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-250 (2ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-250 (2ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
RFX5, regulatory factor X, 5 (influences HLA class II expression), OTTHUMP00000196318, regulatory factor X, 5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Kong X, Fang M, Li P, Fang F, Xu Y, HDAC2 deacetylates class II transactivator and suppresses its activity in macrophages and smooth muscle cells.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TGDKSSEPSTLSNE
NCBI Gene ID #:
5993
NCBI Official Name:
regulatory factor X, 5 (influences HLA class II expression)
NCBI Official Symbol:
RFX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70kDa
Protein Accession #:
NP_000440.1
Protein GI Number:
71040090
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription,Transcription
SPECIFICITY:
Reported variants represent identical protein: NP_000440.1, NP_001020774.1
Swissprot #:
P48382