RPA1 / RPA70 Antibody

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ProSci
Product Code: PSI-43-362
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-362-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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43-362 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-362 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
RPA1, replication protein A1, 70kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of Mouse Lymph Node. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Lada AG, Waisertreiger IS, Grabow CE, Prakash A, Borgstahl GE, Rogozin IB, Pavlov YI.Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CKSYEDATKITVRSN
NCBI Gene ID #:
6117
NCBI Official Name:
replication protein A1
NCBI Official Symbol:
RPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70 + 80kDa
Protein Accession #:
NP_002936.1
Protein GI Number:
4506583
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction, Stem Cell
Swissprot #:
P27694